Antibody

Rabbit Anti-CD68 Antibody

  • Catalog Number : A02675BS
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General Information

Reactivity Mouse, Rat
Application WB, IF, IHC, ELISA, ICC
Host Rat
Clonality Polyclonal
Conjugate Non-conjugated
Alias D68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger r

Figure :

Sample: Bone (Mouse) Lysate at 40 ug

Primary: Anti-CD68 at 1/300 dilution

Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 for 20 min;

Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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