General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, IF, IHC, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
P40763 |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3. |
| Application |
WB : 1:500-1000 IHC : 1:50-100 IF : 1:100-200 |
|---|
| Figure |
Figure 1 : Western blot analysis of extracts from Hela cells untreated or treated with IFN a using A8852P.
Figure 2 : Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using A8852P (left) or the same antibody preincubated with blocking peptide (right).
Figure 3 : Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using A8852P.
Figure 4 : Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using A8852P. |
|---|
%20Antibody/A8852P%20Phospho-STAT3%20(Y705)%20Antibody-WB-1.jpg)
%20Antibody/A8852P%20Phospho-STAT3%20(Y705)%20Antibody-IHC-1.jpg)
%20Antibody/A8852P%20Phospho-STAT3%20(Y705)%20Antibody-IHC-2.jpg)
%20Antibody/A8852P%20Phospho-STAT3%20(Y705)%20Antibody-IF-1.jpg)
| Purification Method | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy. |
|---|---|
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|---|
| Alias | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 |
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