General Information
Reactivity | Human, Mouse, Rabbit |
---|---|
Application | WB, IF, IHC, ELISA |
Host | Rabbit |
Clonality | Polyclonal |
Conjugate | Non-conjugation |
Product Type | Primary Antibody |
---|---|
Uniprot No. |
P40763 |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3. |
Application |
WB : 1:500-1000 IHC : 1:50-100 IF : 1:100-200 |
---|
Figure |
Figure 1 : Western blot analysis of extracts from Hela cells untreated or treated with IFN a using A8852P.
Figure 2 : Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using A8852P (left) or the same antibody preincubated with blocking peptide (right).
Figure 3 : Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using A8852P.
Figure 4 : Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using A8852P. |
---|
Purification Method | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy. |
---|---|
Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
---|
Alias | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 |
---|