Antibody

RAD51 Antibody

  • Catalog Number : A0117P
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General Information

Reactivity Human, Mouse
Application WB, IF, IHC, ELISA, IP
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

Q06609

Immunogen

Recombinant human DNA repair protein RAD51 homolog 1 protein (1-228AA)
Application

WB : 1:2000-5000

IHC : 1:20-200

IF : 1:50-200

Figure

Figure 1 : Western Blot analysis using RAD51 antibody at 2.7ug/ml
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate and K562 whole cell lysate
Secondary Goat polyclonal to rabbit IgG at 1:50000 dilution
Predicted band size: 37, 27, 32 kDa
Observed band size: 37 kDa

Figure 2 : Immunoprecipitating of RAD51 in K562 whole cell lysate

For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:50000)
Lane 1: Rabbit monoclonal IgG(1ug) in K562 whole cell lysate.
Lane 2: RAD51 Antibody (4ug)+ K562 whole cell lysate (500ug)
Lane 3: K562 whole cell lysate (20ug)

 

Figure 3 : Immunohistochemistry of paraffin-embedded human liver cancer using A0117P at 1:100 dilution

 

Figure 4 : Immunofluorescent analysis of Hela cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Purification Method >95%, Protein G purified
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias  DNA repair protein RAD51 homolog 1 (HsRAD51) (hRAD51) (RAD51 homolog A), RAD51, RAD51A RECA

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