Histone H2AG Antibody
- Catalog Number : A0519P
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Introduction
General Information
Application | WB, IF, IHC, ELISA |
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Clonality | Polyclonal |
Conjugate | Non-conjugation |
Uniprot | P0C0S8 |
Isotype | IgG |
Immunogen | Recombinant Human Histone H2A type 1 protein (2-130AA) |
Purity | >95%, Protein G purified |
Storage buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Storage instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Alias | Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM, H2AFP; H2AFC; H2AFD; H2AFI; H2AFN |
Western blot All lanes: HIST1H2AG antibody at 4µg/ml + HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
Immunohistochemistry of paraffin-embedded human lung cancer using CSBPA010389LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSBPA010389LA01HU at dilution of 1:100
Chromatin Immunoprecipitation Hela(1.2*106 )were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010389HU) against the PGK1 promoter. Immunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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