Antibody

VIM Antibody

  • Catalog Number : A0520P
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General Information

Reactivity Human
Application WB, IF, ELISA
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

P08670

Immunogen

Recombinant human Vimentin protein (2-466AA)
Application

WB : 1:1000-5000

IHC : 1:20-200

IF : 1:50-200

Figure

Figure 1 : Immunofluorescent analysis of HepG2 cells using A0520P at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

 

Figure 2 : Immunohistochemistry of paraffin-embedded human endometrial cancer at dilution of 1:100.

 

Figure 3 : Immunohistochemistry of paraffin-embedded human endometrial cancer using A0520P at dilution of 1:100.

 

 

Figure 4 :

Western blot anaylsis using VIM antibody at 8 ug/ml
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A549 whole cell lysate

Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

 

 

 

Purification Method >95%, Protein G purified
Storage Buffer Preservative: 0.03% Proclin 300 
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias 
Vimentin, VIM

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