Porcine cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit

General Information

Reactivity	Porcine
Application	ELISA
Assay principle	This ELISA kit uses the Sandwich ELISA principle . The micro ELISA plate provided in this kit has bee n pre coated with an antibody specific to Porcine cTn I/TNNI3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine cTn I/TNNI3 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine cTn I/TNNI3 , biotinylated detection antibody and Avidin HRP conjugate will appear blue in color. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine cTn I/TNNI3 . You can calculate the concentration of Porcine cTn I/TNNI3 in the samples by comparing the OD of the samples to the standard curve.
Sensitivity	18.75pg/mL
Detection range	31.25 2000pg/mL
Storage instruction	4˚C or -20 ˚C for months

CONTENTS and STORAGE