Protein Biology, Protein ladders
AceColor™ prestained Protein marker (25-400 kDa)
- Catalog Number : A1058
- Number : A1058
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Introduction
This ladder is ideal for determining protein molecular weights during SDS-PAGE and Western blotting. The broad range of molecular weights facilitates dynamic monitoring of protein separation, clear visualization of protein bands, and accurate estimation of target protein sizes. After electrophoresis and transfer onto PVDF or nitrocellulose membranes, the colored protein bands allow easy assessment of transfer efficiency during Western blot experiments. Recommended loading volumes are 5–10 μL per well.
General Information
Important Information：
This product is supplied as a convenient, ready-to-use formulation. It does not require heating, dilution, or the addition of reducing agents.
1. For Western blotting applications, larger proteins (>100 kDa) may require extended transfer times or increased
current for effective transfer. It is recommended to avoid adding SDS to the transfer buffer. If SDS must be used,
keep the concentration below 0.05%.
2. Prestained protein bands may exhibit different mobilities in various SDS-PAGE buffer systems.
In low-percentage gels (<10%), low-molecular-weight proteins may migrate close to the dye front; their positions
should be considered as approximate references when estimating target protein sizes.
3. It is recommended to perform electrophoresis using either a 12% separating gel or a 4–20% gradient gel.
Preparation：
1. This product is provided as a ready-to-use formulation. Prior to loading, thaw at room temperature for several minutes
and gently mix to ensure homogeneity.
(Do not boil, dilute, or add reducing agents!)
2. Load 5 μL of the ladder into each gel well for SDS-PAGE. If wells are wider, the loading volume may be slightly
increased as needed.
It is recommended for first-time users to perform preliminary experiments to determine optimal loading volumes
tailored to their specific experimental conditions and preferences. This practice helps to achieve high-quality
results while minimizing reagent consumption.
3. For regular use, store the product at 2–8°C for up to 3 months. Aliquoting is advised to avoid repeated freeze-thaw
cycles.
Migration patterns of Prestained Protein Ladder：
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis.
Product Use Limitation
For Research Use Only. Not for Diagnostic Purposes.
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