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Gram Staining Kit
- Catalog Number : C6001
- Number : C6001
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Introduction
The Gram Stain is a different staining technique most widely applied in microbiology. Gram staining is based on the ability of bacteria cell wall to retaining the crystal violet dye during solvent treatment. The cell walls for Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gramnegative bacteria Bacteria cell walls are stained by the Gram's Crystal Violet Solution. Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed easily.However, subsequent treatment with Gram's Decolorizing Solution dissolves the lipid layer from the gram-negative cells. As a result, the diffusion of the violet-iodine complex is blocked, and the bacteria remain stained. The length of the decolorization is critical in differentiating the gram-positive bacteria from the gram-negative bacteria. Finally, counterstain with Gram's Safranin Solution, the gram-negative bacteria is stained a pink color.
General Information
| Storage instruction | 2-8 °C, avoid light, valid for 1 year. |
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| Alias | Gram dyeing liquid gram's dye kit |
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