Total Amino Acids (T-AA) Colorimetric Assay
- Catalog Number : AS1002
- Number :
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Size:
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Introduction
General Information
| Assay principle | Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm. |
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| Sensitivity | 3.03 mmol/L |
| Detection range | 3.64-100 mmol/L |
| Sample type | Serum (plasma) ,urine and tissue |
| Storage instruction | 2-8℃ , 6 months |
PRINCIPLE of KIT
Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.
|
Item |
Component |
Specification |
Storage |
|
Reagent 1 |
Powder A |
Powder × 1 vial |
2-8℃ , 6 months |
|
Reagent 2 |
Acid Reagent |
0.8 mL × 1 vial |
2-8℃ , 6 months |
|
Reagent 3 |
Powder B |
Powder × 1 vial |
2-8℃ , 6 months |
|
Reagent 4 |
Powder C |
Powder × 1 vial |
2-8℃ , 6 months |
|
Reagent 5 |
Protein Precipitator |
15 mL × 1 vial |
2-8℃ , 6 months |
|
|
Microplate |
96 wells |
No requirement |
|
|
Plate Sealer |
2 pieces |
|
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Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. |
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