ELISA, COVID-19 ELISA Kit, COVID-19 Neutralizing Antibody
SARS-CoV-2 Neutralization Antibody ELISA Kit
- Catalog Number : E5038
- Number : E5038
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Introduction
General Information
| Reactivity | Human |
|---|---|
| Application | ELISA, Neutralization, in vivo/vitro neutralization |
| Assay principle | This Test kit uses Competitive-ELISA as the method to qualitatively detect the AntiSARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRPRBD). After 37℃ incubation, the unbound HRP-RBD, as well as any HRP-RBD bound to non-Neutralization antibody, will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRPRBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not. |
| Sample type | human serum or plasma |
| Storage instruction | 6 months at 2-8℃ |
CONTENTS and STORAGE
|
Item |
Specifications |
Storage |
|
Micro ELISA Plate |
24T: 8 wells ×3 strips 96T: 8 wells ×12 strips 96T*5: 5 plates, 96T |
2-8℃, 1 week |
|
Positive Control |
24T: 1 vial 96T: 2 vials 96T*5: 10 vials |
2-8℃, 6 months |
|
Negative Control |
24T: 1 vial 96T: 2 vials 96T*5: 10 vials |
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Concentrated HRP Conjugated RBD (HRP-RBD, 100×) |
24T: 1 vial, 60 μL 96T: 1 vial, 120 μL 96T*5: 5 vials, 120 μL |
2-8℃(Protect from light), 6 months |
|
Sample & Control Diluent |
24T/96T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃, 6 months |
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HRP Conjugated RBD Diluent |
24T/96T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
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Concentrated Wash Buffer(25×) |
24T/96T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
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Substrate Reagent |
24T/96T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
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Stop Solution |
24T/96T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃, 6 months |
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Plate Sealer |
24T/96T: 3 pieces 96T*5: 15 pieces |
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Product Description |
1 copy |
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Certificate of Analysis |
1 copy |
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