McCoy's 5A Dry Powder Media
- Catalog Number : CM1043
- Number : CM1043
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Size:
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Introduction
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, mcCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with growth factors, Fetal Bovine Serum (FBS), Specsure® Bovine Growth Serum or serum-free additives. McCoy's 5A Dry Powder Media does not contain sodium bicarbonate, therefore adding 2.2 g sodium bicarbonate is needed for a 5% CO2 environment to maintain physiological pH.
General Information
| Storage instruction | 2-8 ˚C |
|---|
- Sterility：Sterile-filtered
- Form：Powder
- Application (s)：Cell culture/ mammalian: suitable
- Impurities：Endotoxin, tested
- Components：
HEPES (-)
Sodium bicarbonate (-)
L-glutamine (1.5 mM)
D-glucose (3000 mg/L)
Phenol red (10 mg/l)
Sodium pyruvate (-)
NOTES
- 1L McCoy's 5A medium can be prepared.
- Dissolve the McCoy's 5A Powder with ultrapure water for cell culture.
- Add 2.2 g sodium bicarbonate and dissolve fully.
- (Optional) Adjust pH with 1M NaOH solution or 1M HCl solution.
- Add ultrapure water for cell culture to the final volume of 1L.
- The prepared solution should be sterilized with 0.22 µm filters under aseptic condition.
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