Protein Biology, COVID-19 
, COVID-19 (SARS-CoV-2) proteins
SARS-CoV-2 (2019-nCoV) S-trimer Protein (Mammalian, D614G, C-6His)
- Catalog Number : P0132
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Introduction
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
General Information
| Uniprot | Accession: QHD43416.1 |
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| Purity | >95%. Affinity purification chromatography. |
| Molecular Weight | 136.6kDa |
| Formula | Supplied as a 0.2 μm filtered solution of PBS, PH 7.4. |
| Storage instruction | Reconstituted protein solution should be stored at ≤ -20°C. |
| Alias | 2019-nCov S protein; 2019-nCoV Spike glycoprotein; 2019-nCoV S glycoprotein |
Quality Control:
Mol Mass: 136.5kDa AP Mol Mass: 170-220kDa, reducing conditions
Purity: Greater than 95% as determined by reducing SDS-PAGE.
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Purity: Greater than 90% as determined by reducing SEC-HPLC. |
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Purity: Greater than 90% as determined by reducing SEC-HPLC. |
Bioactivity:
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Immobilized SARS-CoV-2 (2019-nCoV) S-trimer Protein (Mammalian, D614G, C-6His) (Cat#P0132) at 2μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#C05Y). The ED50 of Recombinant Human ACE-2-Fc is 31.75 ng/ml. |
Immobilized SARS-CoV-2 (2019-nCoV) S-trimer Protein (Mammalian, D614G, C-6His) (Cat#P0132) at 2μg/ml (100 μl/well) can bind Anti-2019-nCoV S1 mAb (5D9). The ED50 of Anti-2019-nCoV S1 mAb (5D9) is 15.81 ng/ml. |
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