Protein Biology, COVID-19 
, COVID-19 (SARS-CoV-2) proteins
SARS-CoV-2 (2019-nCoV) S-trimer Protein (Mammalian, C-6His)
- Catalog Number : P0131
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Introduction
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
General Information
| Uniprot | Accession: QHD43416.1 |
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| Purity | >95%. Affinity purification chromatography. |
| Molecular Weight | 136.6kDa |
| Formula | Supplied as a 0.2 μm filtered solution of PBS, PH 7.4. |
| Storage instruction | Reconstituted protein solution should be stored at ≤ -20°C. |
| Alias | 2019-nCov S protein; 2019-nCoV Spike glycoprotein; 2019-nCoV S glycoprotein |
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SDS-PAGE: |
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SEC-MALS: |
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Greater than 95% as determined by reducing SDS-PAGE. |
SARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) molecular mass was tested by SEC-MALS around 550-670 kDa and the purity was more than 90%. |
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Bioactivity-Elisa: |
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ImmobilizedSARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) at 2μg/ml (100 μl/well) can bind Human ACE-2 (C-Fc)(Cat#C05Y). The ED50 for this effect is 15-120 ng/ml. |
Immobilized SARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) at 2μg/ml (100 μl/well) can bind Anti-2019-nCoV S1 mAb (5D9)(Cat#NC025). The ED50 for this effect is 5-30 ng/ml. |
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Bioactivity-BLI: |
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Loaded Human ACE-2-Fc on Protein A Biosensor, can bind SARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) with an affinity constant of 0.125 nM as determined in BLI assay. |
Loaded Anti-2019-nCoV S1 mAb on Protein A Biosensor, can bind SARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) with an affinity constant of 3.88 nM as determined in BLI assay. |
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LoadedSARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) on HIS1K Biosensor, can bind Anti-2019-nCoV S-hIgG1 Antibody (8A5) with an affinity constant of 1.11 nM as determined in BLI assay. |
Product stability:
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Bind with Recombinant Human ACE-2 (C-Fc)
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Bind with Anti-2019-nCoV S1 mAb (5D9)
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Stability testing is performed in the product buffer to see whether different temperatures affect the antigen binding.The result showed that product bioactivity is no significant differences after storage for 14 days at 4℃ or 25℃. |
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Flow cytometry:
SARS-CoV-2 (2019-nCoV) S-trimer Protein (C-6His)(Cat#P0131) can bind 293-ACE2 Overexpressed Cells (Cat#CL0021). The percentage for positive cell is 92.1%.
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