Urea Colorimetric Assay Kit
- Catalog Number : AS1001
- Number : AS1001
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Introduction
This kit can be used to measure urea content in animal serum, plasma, urine, saliva, milk samples.
Detection significance:
Urea is the major final-product of protein metabolism in the body, which constitutes the clear majority of non-protein nitrogen in blood. Blood urea nitrogen come from the liver, which excreted with urine through kidney. Renal function failure, nephritis, urinary tract obstruction and so on can make the content of blood urea increased. Urea is the largest nitrogen circulating sediment except the nitrogen in circulating protein, and it is also the main carrier of removing harmful ammonia in the body.
Detection significance:
Urea is the major final-product of protein metabolism in the body, which constitutes the clear majority of non-protein nitrogen in blood. Blood urea nitrogen come from the liver, which excreted with urine through kidney. Renal function failure, nephritis, urinary tract obstruction and so on can make the content of blood urea increased. Urea is the largest nitrogen circulating sediment except the nitrogen in circulating protein, and it is also the main carrier of removing harmful ammonia in the body.
General Information
| Assay principle | Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with amphyl and form a green substance in alkaline medium, and the production of the green substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm. |
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| Sensitivity | 0.09 mmol/L |
| Detection range | 0.28-35 mmol/L |
| Storage instruction | Store at 2-8˚C for 6 month |
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