Chemical, Buffer, Western Blotting, Buffer
Ponceau S solution
- Catalog Number : A1052
- Number : A1052-50ml
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Introduction
It is commonly used for the detection of protein on PVDF membrane, nitrocellulose membrane and cellulose acetate membrane; immerse the PVDF membrane, nitrocellulose membrane or cellulose acetate membrane with protein in the Ponceau staining solution and shake for 5-10 Minutes or more, after the protein bands appear on the membrane, take out the blotting membrane and rinse with distilled water, PBS or other appropriate solution for 2-3 times, each time for 3-5 minutes, until the red background disappears, the subsequent follow-up WB detection.
1. Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid + 3 % sulphosalicylic acid, incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.
2. Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid, incubate 1-5 mins. at room temperature. Ponceau S stained bands on PVDF only appear bright and distinct after drying.
1. Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid + 3 % sulphosalicylic acid, incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.
2. Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid, incubate 1-5 mins. at room temperature. Ponceau S stained bands on PVDF only appear bright and distinct after drying.
General Information
| Application | WB, Chemical |
|---|---|
| Cas No. | 6226-79-5 |
| Molecular Weight | 760.6 |
| Formula | C22H12N4Na4O13S4 |
| Storage instruction | Store at 2 to 8℃ |
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