ACE Scipt II One Step RT-PCR Kit (Dye Plus)
- Catalog Number : EP2007
- Number : EP2007
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Size:
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Introduction
The ACE Scipt II One Step RT-PCR Kit (Dye Plus) is specially designed for RNA detection (such as RNA virus). With the ACE Scipt II One Step RT-PCR Kit (Dye Plus) and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The ACE Scipt II One Step RT-PCR Kit (Dye Plus)contains an opimized buffer, dNTPs, and Loading Dye.
General Information
| Application | RT |
|---|---|
| Storage instruction | All components should be stored at -20℃ |
CONTENTS
|
Component |
EP2007 50 rxn (50 μl/rxn) |
|
RNase free ddH2O 2× One Step Mix (Dye Plus)a One Step Enzyme Mixb |
1 ml × 2 625μl × 2 125μl |
a. Contains dNTPs and Loading Dye.
b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.
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