Introduction
This kit allows for the determination of Cortisol concentrations in Porcine serum, cell culture supernates and other biological fluids
General Information
| Application | ELISA |
|---|---|
| Host | Procine |
| Assay principle | The kit assay Porcine Cortisol level in the sample, use Purified Porcine Cortisol antibody to coat microtiter plate wells, make solid-phase antibody, then add Cortisol to wells, Combined Cortisol antibody which With HRP labeled,become antibody - antigen – enzymeantibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Porcine Cortisol in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Sensitivity | 1.75 μg/L |
| Detection range | 7-200 μg/L |
| Storage instruction | Store at 2-8˚C for 6 month |
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