ELISA

Porcine Cortisol ELISA Kit

  • Catalog Number : E5020
  • Number : E5020
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Introduction

This kit allows for the determination of Cortisol concentrations in Porcine serum, cell culture supernates and other biological fluids

General Information

Application ELISA
Host Procine
Assay principle The kit assay Porcine Cortisol level in the sample, use Purified Porcine Cortisol antibody to coat microtiter plate wells, make solid-phase antibody, then add Cortisol to wells, Combined Cortisol antibody which With HRP labeled,become antibody - antigen – enzymeantibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Porcine Cortisol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sensitivity 1.75 μg/L
Detection range 7-200 μg/L
Storage instruction Store at 2-8˚C for 6 month

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