Mbo II
- Catalog Number : RE0471
- Number :
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Size:
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General Information
| Storage buffer | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
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Recognition site |
GAAGA(N)8↑ |
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Source |
An E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis |
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Assayed on |
Lambda DNA (dam-) |
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Unit definition |
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl. |
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Optimal SE-buffer |
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
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Enzyme activity (%) |
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