Protein Biology, Western Blotting, Protein Marker, Electrophoresis, Protein ladders
AceColor™ Prestained Protein Marker (8.5-180 kDa)
- Catalog Number : A1053
- Number : A1053
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Introduction
ACE Biolabs AceColor™ Prestained Protein Ladder is a mixture of 10 blue-, a orange-, and a green-stained proteins (8.5 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. Molecular Weight: 180, 130, 95, 72(orange), 55, 43, 34, 25, 17, 8.5(green) kDa.
The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
General Information
Applications
- Monitoring protein migration during SDS-PAGE.
- Monitoring protein transfer efficiency on membranes after Western blot transferring.
- Sizing estimation of proteins on SDS-PAGE and Western blots.
PROTOCOL
- For SDS-PAGE: Loading 3 μl or 5 μl per well for clear monitoring electrophoresis on a 15-well or 10-well mini-gel. Apply more for a thicker (> 1.5 mm) or larger gel
- For Western blotting: Loading 1.5~2.5 μl per well for monitoring electrophoresis and general Western blot transferring efficiency. Apply more for a thicker (> 1.5 mm) or larger gel
Note: Do NOT heat, dilute, or add reducing agents before loading.
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PRODUCT USE LIMITATION
These products are intended for research use only.
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