M-199 Dry Powder Media
- Catalog Number : CM1021、CM1022
- Number : CM1021
-
Size:
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Introduction
M199 (Media 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic Media, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in CO2 environment, hank's salt is used in non-CO2 environment. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives. Serum supplements are required before use, usually with 10% Fetal Bovine Serum (FBS) or Specsure® Bovine Growth Serum. Uses sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5% CO2 environment to maintain physiological pH.
General Information
| Cas No. | CM1021、CM1022 |
|---|
- Sterility：Sterile-filtered
- Form：Powder
- Application (s)：Cell culture/ mammalian: suitable
- Impurities：Endotoxin, tested
- Components：
HEPES (-)
L-glutamine (+)
D-glucose (1000 mg/ml)
Phenol red (+)
Sodium bicarbonate (-) - Storage temp：2-8 ˚C
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