Phospho-Akt Pathway Antibody Sampler Kit

Catalog Number : AK0220
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Introduction

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

General Information

Reactivity	Human, Mouse, Rabbit
Application	WB, ELISA
Host	Rabbit
Clonality	Polyclonal
Conjugate	HRP,Non-conjugated
Storage instruction	Store at -20℃. Avoid freeze / thaw cycles.
Research topic	AKT/PKB Signaling Pathway

PRODUCT INCLUDES

Cat No.	Product name	Quantity	Applications	Reactivity	Host
A340420	Pan-Akt Polyclonal Antibody	20μL	WB, IHC, ELISA	Human, Mouse, Rat	Rabbit
A340365	Phospho-AKT1 (Ser473) Polyclonal Antibody	20μL	WB, IHC, IF, ELISA	Human, Mouse, Rat	Rabbit
A340330	Phospho-Pan-Akt (Thr308) Polyclonal Antibody	20μL	WB, IHC, ELISA	Human, Mouse, Rat	Rabbit
A340282	Phospho-RAF1 (Ser259) Polyclonal Antibody	20μL	WB, IHC, ELISA	Human, Mouse, Rat, Monkey	Rabbit
A340230	Phospho-GSK3 alpha/beta (Tyr279/216) Polyclonal Antibody	20μL	WB, IHC, ELISA	Human, Mouse, Rat	Rabbit
A340231	Phospho-GSK3 beta (Ser9) Polyclonal Antibody	20μL	WB, IHC, IP, ELISA	Human, Mouse, Rat	Rabbit
A340281	Phospho-PTEN (Ser370) Polyclonal Antibody	20μL	WB, IHC, ELISA	Human, Mouse, Rat	Rabbit
A1013s	Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated)	120μL	WB, ELISA	Rabbit	Goat

Phospho-Akt Pathway Antibody Sampler Kit

Introduction

General Information

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Phospho-Akt Pathway Antibody Sampler Kit

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