Antibody, Antibody Sampler Kit
mTOR Substrates Antibody Sampler Kit
- Catalog Number : AK0195
- Number :
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Size:
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Introduction
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy. The regulatory associated protein of mTOR (Raptor) interacts with mTOR to mediate mTOR signaling to downstream targets. Raptor binds to mTOR substrates, such as 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated substrate phosphorylation. Binding of the FKBP12-rapamycin complex to mTOR inhibits mTOR-raptor interaction, which suggests a mechanism for the inhibition of mTOR signaling by rapamycin. This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin are dependent on a protein called GβL. GβL is part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR) and may mediate rictor-mTOR signaling to PKCα and other downstream targets. The rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB at Ser473, which is required for PDK1 phosphorylation of Akt/PKB at Thr308 and full activation of Akt/PKB.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA, IHC |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | HRP,Non-conjugated |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | mTOR signaling pathway |
PRODUCT INCLUDES
|
Cat No. |
Product name |
Quantity |
Applications |
Reactivity |
Host |
|
A340257 |
Phospho-MTOR (Ser2448) Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340598 |
MTOR Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340616 |
p70 S6 kinase alpha Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340345 |
Phospho-p70 S6 kinase alpha (Ser371) Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A1013s |
Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) |
120μL |
WB, ELISA |
Rabbit |
Goat |
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