Introduction
General Information
| Reactivity | Bovine |
|---|---|
| Application | ELISA |
PRINCIPLE of KIT
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bovine IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for bovine IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of bovine IgG in samples. The color development is stopped and the intensity of the color is measured.
SPECIFICATION
Sensitivity: 7.397 μg/mL.
Detection Range: 9.4– 600 μg/mL
Specificity: This kit recognizes bovine IgG in samples. No significant cross-reactivity or interference between mouse IgG and analogues was observed.
Intra-assay Precision (Precision within an assay): CV% <8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% <10%
Three samples of known concentration were tested in twenty assays to assess.
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