T7 High Yield RNA Transcription Kit

Catalog Number : EP5001
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General Information

Charactistics	High yield - Produces 150-200 µg RNA from 0.5 µg template in 20 µl reaction solution. Flexible - Generates unlabeled, biotin-labeled or dye-labeled RNA. Optimized reaction system for in vitro transcription T7 High Yield RNA Transcription Kit contains T7 RNA Polymerase Mix, 10X Reaction Buffer, ATP Solution, UTP Solution, GTP Solution, CTP Solution, DNase I, Control Template (0.5 μg/μl), and Nuclease-free Water.

Charactistics

High yield - Produces 150-200 µg RNA from 0.5 µg template in 20 µl reaction solution.
Flexible - Generates unlabeled, biotin-labeled or dye-labeled RNA.
Optimized reaction system for in vitro transcription
T7 High Yield RNA Transcription Kit contains T7 RNA Polymerase Mix, 10X Reaction Buffer, ATP Solution, UTP Solution, GTP Solution, CTP Solution, DNase I, Control Template (0.5 μg/μl), and Nuclease-free Water.

Figures	Figure 1. Transcription reaction catalyzed by T7 RNA polymerase Figure 2. Accumulation curve of RNA transcript in standard reaction with 3 different size of templates. Figure 3. Accumulation of RNA transcript in strandard transcription reaction with different amount of 0.8 kb PCR product as templates after 2 hours incubation.

Figures

Figure 1. Transcription reaction catalyzed by T7 RNA polymerase

Figure 2. Accumulation curve of RNA transcript in standard reaction with 3 different size of templates.

Figure 3. Accumulation of RNA transcript in strandard transcription reaction with different amount of 0.8 kb PCR product as templates after 2 hours incubation.

T7 High Yield RNA Transcription Kit

General Information

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