ELISA

Rat Hepc(Hepcidin) ELISA Kit

  • Catalog Number : E0005
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Introduction

This ELISA kit applies to the in vitro quantitative determination of Rat Hepc concentrations in serum, plasma and other biologic

General Information

Reactivity Rat
Application ELISA

PRINCIPLE of KIT

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Hepc. You can calculate the concentration of Rat Hepc in the samples by comparing the OD of the samples to the standard curve.


SPECIFICATION

Sensitivity: 46.88 pg/mL

Detection Range: 78.13-5000 pg/mL

Specificity: This kit recognizes Rat Hepc in samples. No Significant cross-reactivity or interference between Rat Hepc

and analogues was observed.

Repeatability: Coefficient of variation is < 10%.

Sample Volume Required Per Well: 100μL

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