Introduction
General Information
| Reactivity | Human |
|---|---|
| Application | ELISA |
PRINCIPLE of KIT
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated wit Human BK. During the reaction, Human BK in the sample or standard competes with a fixed amount of Human BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BK in the samples is then determined by comparing the OD of the samples to the standard curve.
SPECIFICATION
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Specificity: This kit recognizes Human BK in samples. No Significant cross-reactivity or interference between Human
BK and analogues was observed.
Repeatability: Coefficient of variation is < 10%.
Sample Volume: 50μL
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