Primary Antibody

Akt (phospho Ser473) Polyclonal Antibody

  • Catalog Number : A20650PI
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General Information

Reactivity Human, Mouse, Rabbit
Application WB, IF, IHC, ELISA
Host Rabbit
Uniprot P31749
Immunogen Synthesized phospho-peptide around the phosphorylation site of human Akt (phospho Ser473)
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formula Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Phospho-Akt (S473) Polyclonal Antibody diluted at 11000


Western Blot analysis of MCF7 cells using Phospho-Akt (S473) Polyclonal Antibody diluted at 11000

 


Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

 

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