Primary Antibody

GFAP Monoclonal Antibody(5C8)

Catalog Number : A20465PI
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Introduction

glial fibrillary acidic protein(GFAP) Homo sapiens This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by Ref Seq, Oct 2008],

General Information

Reactivity	Human, Mouse, Rabbit
Application	WB, IF, IHC
Host	Mouse
Clonality	Monoclonal
Conjugate	Non-conjugation
Uniprot	Human: P14136 / Mouse: P03995/ Rat: P47819
Immunogen	Synthetic Peptide of GFAP
Assay principle	WB：1:2000-1:5000/IHC：1:50-1:300/IF：1:200
Purity	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Formula	PBS, pH ₇.₄, containing ₀.₅%BSA, ₀.₀₂% sodium azide as Preservative and ₅₀% Glycerol.
Storage instruction	Store at -20℃ for 1 year.
Alias	GFAP; Glial fibrillary acidic protein; GFAP

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mousekidney tissue. 1,GFAP Monoclonal Antibody(5C8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Immunofluorescence analysis of Rat-brain tissue. 1,GFAP Monoclonal Antibody(5C8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Western blot analysis of Rat Brain Tissue, diluted at 1:5000.

Western blot analysis of lysates from 1) Rat Brain Tissue, 2)HeLa , 3)A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23910)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Polyclonal Antibody (cat:YT4780) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23720)was diluted at 1:10000, 37° 1hour.

GFAP Monoclonal Antibody(5C8)

Introduction

General Information

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GFAP Monoclonal Antibody(5C8)

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