α-tubulin Monoclonal Antibody(8F11)
- Catalog Number : A20446PI
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General Information
| Reactivity | Human, Mouse, Rabbit |
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| Application | WB, IF, IHC, IP |
| Host | Mouse |
| Uniprot | Q71U36/P68363 |
| Immunogen | Recombinant Protein of Tubulin alpha-1A chain |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Formula | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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Figure :
Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue, diluted at 1:5000.
1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1:200
Immunofluorescence analysis of Human-colon-cancer tissue. 1,α-tubulin Monoclonal Antibody (8F11) (red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1,α-tubulin Monoclonal Antibody (8F11) (red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,α-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,α-tubulin Monoclonal Antibody(8F11) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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