RB1 Antibody
- Catalog Number : A20359PI
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General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, IF, IHC, ELISA |
| Host | Rabbit |
| Uniprot | P06400 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoblastoma. AA range:751-800 |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Formula | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Alias | RB; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
|
Figure:
Immunofluorescence analysis of rat-lung tissue. 1,Rb Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,Rb Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
mmunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of various cells using Rb Polyclonal Antibody diluted at 1：1000. |
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