| Type |
E. coli. expression vector |
| Antibiotic selection |
Ampicillin |
| Map |
 |
| Note |
The folding properties of the MW 14000 synthetic IgG binding domains do not interfere with the folding of the protein fused to them. Hosts: E.coli JM105. |
LOCUS pEZZ18 4591 bp DNA SYN 21-Nov-2013
DEFINITION pEZZ18
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4591
/organism="pEZZ18"
/mol_type="other DNA"
promoter 131..159
/label="AmpR_promoter"
CDS 201..1061
/label="Ampicillin"
/gene="Ampicillin"
/note="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
gene 201..1061
/label="Ampicillin"
/gene="Ampicillin"
rep_origin 1216..1835
/label="pBR322_origin"
promoter 2144..2173
/label="lac_promoter"
misc_feature 2187..2209
/label="M13_pUC_rev_primer"
misc_feature 2208..2226
/label="M13_reverse_primer"
CDS 2396..3157
/label="ORF frame 2"
/translation="MMTLQIHTGGINLKKKNIYSIRKLGVGIASVTLGTLLISGGVTP
AANAAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEA
KKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEA
KKLNDAQAPKVDANSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLAAHP
PFASWRNSEEARTDRPSQQLRSLNGEWRFRCNGWR*"
misc_feature complement(2974..2990)
/label="M13_forward20_primer"
misc_feature complement(2983..3005)
/label="M13_pUC_fwd_primer"
misc_feature 2983..3126
/label="lacZ_a"
rep_origin 3448..3754
/label="f1_origin"
misc_feature 4541..4563
/label="pGEX_3_primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
2221 accatgatta cgaattagcg gccgctagcc gaaatagcgt gattttgcgg ttttaagcct
2281 tttacttcct gaataaatct ttcagcaaaa tatttatttt ataagttgta aaacttacct
2341 ttaaatttaa ttataaatat agattttagt attgcaatac ataattcgtt atattatgat
2401 gactttacaa atacatacag ggggtattaa tttgaaaaag aaaaacattt attcaattcg
2461 taaactaggt gtaggtattg catctgtaac tttaggtaca ttacttatat ctggtggcgt
2521 aacacctgct gcaaatgctg cgcaacacga tgaagccgta gacaacaaat tcaacaaaga
2581 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
2641 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
2701 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
2761 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
2821 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
2881 gctaaatgat gctcaggcgc cgaaagtaga cgcgaattcg agctcggtac ccggggatcc
2941 tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga
3001 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
3061 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
3121 tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat attaccagca
3181 aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta
3241 ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt
3301 ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc
3361 tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag
3421 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
3481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
3541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
3601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
3661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
3721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
3781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
3841 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat
3901 acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg
3961 ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac
4021 ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag
4081 ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt
4141 ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa
4201 atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg
4261 tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt
4321 ctttgccttg cctgtatgat ttattggatg ttggaattga tgcggtattt tctccttacg
4381 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
4561 aggttttcac cgtcatcacc gaaacgcgcg a
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