Antibody

HIF1A Antibody

  • Catalog Number : A8917P
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General Information

Reactivity Human
Application WB, IF, IHC, ELISA
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

Q16665
Immunogen Recombinant human Hypoxia-inducible factor 1-alpha protein (579-826AA)
Application

WB : 1:500-5000

IHC : 1:20-200

IF : 1:50-200

Figure

Figure 1 :  Western blot analysis using A8917P at 2ug/ml
Lane 1: HGC27 whole cell lysate
Lane 2: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 93, 83, 96 kDa
Observed band size: 93 kDa

 

Figure 2 : Immunocytochemistry analysis of human bladder cancer using A8917P at dilution of 1:100

 

Figure 3 : Immunocytochemistry analysis of human colon cancer using A8917P at dilution of 1:100

 

Figure 4 : Immunofluorescent analysis of Hela cells using A8917P at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Purification Method >95%, Protein G purified
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias  Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting protein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loop-helix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8), HIF1A, BHLHE78 MOP1 PASD8

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