General Information
Reactivity | Human |
---|---|
Application | WB, IHC, ELISA |
Host | Rabbit |
Clonality | Polyclonal |
Conjugate | Non-conjugation |
Product Type | Primary Antibody |
---|---|
Uniprot No. |
P40763 |
Immunogen | A synthesized peptide derived from human Phospho-STAT3 (Tyr705) |
Application |
WB : 1:500-5000 IHC : 1:50-200 |
---|
Figure |
Figure 1 : Western Blot anaylsis using Phospho-STAT3 antibody at 0.925ug/ml Lane 2 : Jurkat whole cell lysate (treated with EGF or Pervanadate)
Figure 2 : IHC image of A8851P diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
---|
Purification Method | Affinity-chromatography |
---|---|
Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
---|
Alias | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 |
---|