Antibody

STAT3 Antibody

  • Catalog Number : A8846P
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General Information

Reactivity Human
Application IF, IHC, ELISA, IP
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

P40763
Immunogen Recombinant human Signal transducer and activator of transcription 3 protein (50-240AA)
Application

IHC : 1:20-200

IF : 1:50-500

IP : 1:200-2000

Figure

Figure 1 :  Immunohistochemistry of paraffin-embedded human liver tissue using A8846P at dilution of 1:100

 

Figure 2 : Immunofluorescent analysis of HepG2 cells using A8846P at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

 

Figure 3 :  Immunoprecipitating STAT3 in Hela whole cell lysate 

For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 1: Rabbit control IgG(1ug)instead of A8846P in Hela whole cell lysate.
Lane 2: CSB-PA022812LA01HU(6ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (10ug)

Purification Method >95%, Protein G purified
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias  Signal transducer and activator of transcription 3 (Acute-phase response factor), STAT3, APRF

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