Antibody

CCT5 Antibody

  • Catalog Number : A4489P
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General Information

Reactivity Human
Application WB, IHC, ELISA, IP
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

P48643
Immunogen Recombinant Human T-complex protein 1 subunit epsilon protein (1-270AA)
Application

WB : 1:1000-5000

IHC : 1:20-200

Figure

Figure 1 : Western blot anaylsis using CCT5 antibody at 1.78 µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Colo320 whole cell lysate
Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60, 50 kDa
Observed band size: 60 kDa

 

Figure 2 : Immunohistochemistry of paraffin-embedded human testis tissue using A4489P at dilution of 1:100

 

Figure 3 : Immunohistochemistry of paraffin-embedded human colon cancer using A4489P at dilution of 1:100

 

Figure 4 : Immunoprecipitating CCT5 in Hela whole cell lysate

For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 1: Rabbit control IgG (1µg) in Hela whole cell lysate.
Lane 2: A4489 (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)

Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias  T-complex protein 1 subunit epsilon (TCP-1-epsilon) (CCT-epsilon), CCT5, CCTE KIAA0098

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