Mycoplasma Removal Agent
- Catalog Number : CM1003
- Number : CM1003
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Size:
Qty : - Price : 5000 詢價
- Stock : 4
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Introduction
Our product utilizes a combination of effective components that target mycoplasma ribosomes to inhibit protein synthesis and disrupt cell membrane integrity. It also acts on DNA helicase to inhibit the synthesis and replication of mycoplasma DNA. The ingredients can penetrate mammalian cell membranes, eliminating both extracellular and intracellular mycoplasma, ensuring that treated cells are not re-infected by intracellularly released mycoplasma. Within 1-2 weeks, over 90% of mycoplasma can be eradicated with minimal cytotoxic effects. Additionally, this product exhibits inhibitory effects on both Gram-negative and Gram-positive bacteria.
General Information
| Application | Cell |
|---|---|
| Storage buffer | Store at -20°C. |
| Storage instruction | Avoid freeze-thaw. |
Product Features
- High Efficacy: Exhibits broad-spectrum elimination against multiple mycoplasma strains.
- Rapid Action: Significantly reduces mycoplasma contamination in a short period.
- Safety: Non-toxic to cells, ensuring normal cell growth and function.
- Ease of Use: Simply add to the culture medium without the need for complex procedures.
Application
- Suitable for various cell cultures, including animal cells, human cells, and stem cells.
- Ideal for use in research laboratories, pharmaceutical companies, and biotechnology firms during cell culture
processes.
- Particularly beneficial for precision experiments and research requiring high-purity cell cultures.
Usage Instructions
1. Thawing Instructions: After thawing, if any crystallization occurs, place the vial in a 37°C water bath for
10-15 minutes until completely dissolved. This will not affect the product's efficacy.
2. Preparation of Working Medium: Add the product to the complete culture medium at a 1:100 ratio to
prepare a 1X working medium.
3. Cell Passage Instructions: During cell passaging, centrifuge to remove the culture supernatant, then
resuspend the cells in the 1X working medium for normal culture and passaging
for 1-2 weeks. Washing cells with PBS 1-2 times during passaging can enhance
the efficiency of mycoplasma elimination.
Example of Elimination Effect
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Note: M represents Marker I; 1 is the positive control; 2 is the untreated mycoplasma-infected cells; 3 is the cells after one
week of treatment.
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