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ELISA, COVID-19 
, COVID-19 ELISA Kit
Human Novel Coronavirus (SARS-CoV-2 ) Neutralizing Antibody ELISA Kit
- Catalog Number : E5019
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Introduction
Neutralizing Antibody ELISA Kit is intended for qualitative measurement of neutralizing antibodies in human serum or plasma. SARS-CoV-2 neutralizing antibodies directly target the SARS-CoV-2 S receptor-binding domain (RBD), blocking the protein's interaction with the host ACE2 receptor, thus interfering with the viral attachment to the cell as well as its entry into the cell. When using the reference neutralizing antibody provided in the kit, the assay has a detection range of 9.75-10000 ng/ml, a sensitivity of as low as 9.75 ng/ml, and Intra- /Inter-assay Precision low than 15%. This detection kit is simple in operation and scalable in sample size (high throughput). Multiple validations display has excellent sensitivity and high specificity. It is conducive to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection. And it should not be used for the diagnosis of acute SARS-CoV-2 infection.
The assay employs the competitive inhibition enzyme immunoassay technique as well as a colorimetric reaction, in which the SARS-CoV-2 neutralizing antibodies in the sample and standard compete with the HRP-conjugated ACE2 to bind to the pre-coated SARS-CoV-2 S RBD. After the sequential addition of the TMB Substrate Solution and the Stop solution, the solution color develops in opposite to the amount of SARS-CoV-2 neutralizing antibodies in the sample. The presence of neutralizing antibodies in the sample can be detected by a reduction signal of HRP-ACE2/SARS-CoV-2 S RBD binding.
The assay employs the competitive inhibition enzyme immunoassay technique as well as a colorimetric reaction, in which the SARS-CoV-2 neutralizing antibodies in the sample and standard compete with the HRP-conjugated ACE2 to bind to the pre-coated SARS-CoV-2 S RBD. After the sequential addition of the TMB Substrate Solution and the Stop solution, the solution color develops in opposite to the amount of SARS-CoV-2 neutralizing antibodies in the sample. The presence of neutralizing antibodies in the sample can be detected by a reduction signal of HRP-ACE2/SARS-CoV-2 S RBD binding.
General Information
| Reactivity | Human |
|---|---|
| Application | ELISA |
| Assay principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with RBD. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated ACE2. The competitive inhibition reaction is launched between with HRP-ACE2 and SARS-CoV-2 neutralizing antibody in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of SARS-CoV-2 neutralizing antibody in the sample. The color development is stopped and the intensity of the color is measured. |
| Sensitivity | 9.75 ng/mL. |
| Detection range | 9.75 - 10000 ng/mL. |
| Sample type | Serum, Plasma |
| Storage instruction | Store at 2 - 8°C. Do not use the kit beyond the expiration date. |
| Research topic | COVID-19, Neutralization Antibody |
| Alias | 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2 |
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