pseudovirus-SARS-CoV-2-abMEN

The partial ORF1a/b SEQ.and N gene coding sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2 pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifuge. For the FNV-SARS-CoV-2 pseudovirus we used ultracentrifugation. Viral envelope of the FNV-SARS-CoV-2-abEN pseudovirus include ORF1a/b SEQ.and N gene coding sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.

#PV019
#Pseudovirus-SARS-CoV-2-abMEN
The partial ORF1a/b, M Gene, E gene and N gene coding sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2-abMEN pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifuge. For the FNV-SARS-CoV-2-abMEN pseudovirus we used ultracentrifugation. Viral envelope of the FNV-SARS-CoV-2-abMEN pseudovirus include ORF1a/b, M Gene, E gene and N gene coding sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.

Formula: >1 x 107 copy/ml in 1 ml
Tag Info: ORF1a/b SEQ, E Gene & N Gene
Storage buffer: glucose、KH2PO4、Na2HPO4、NaCl、KCl、FNV-SARS-CoV-2-abMEN pseudovirus
Storage instruction:Store at -20°C for 6 months

1. ORF1 a/b sequence

ATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCA

AATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTAC

AAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTAC

ACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAA

GGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGC

TTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGC

GGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCA

CTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAA

TGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAAT

TGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAA

TTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAA

CTACCAACATGAAGAAACAATTTATAATTTACTTAAGGAT

TGTCCAGCTGTTGCTAAACAT



2. E Gene

ATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG

TTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTT

GCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGT

GCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAAC

CTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTC

TTCTAGAGTTCCTGATCTTCTGGTCTAA



3.N Gene

ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCC

GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAA

CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGT

CGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCA

CCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC

TCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCA

GATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAA

TTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAG

ATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGA

CTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTG

CAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGG

CACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAA

CTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAG

AAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTC

ATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGC

AGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATG

GCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATT

GAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAA

CAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGG

CTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGC

ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAA

CAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGAC

AAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATT

TGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATT

GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACA

CAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAA

AGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATAC

AAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGA

AGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAA

GAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTG

GATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTG

CTGACTCAACTCAGGCCTAA



4.M Gene

GGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAA

AAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTAT

TCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAA

CAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTC

TGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTG

CTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGC

TATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGC

TACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTT

CCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAA

CGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTA

GAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGAC

ATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACAT

CAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGA

ACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAG

CAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGAT

TGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGT

GACAATATTGCTTTGCTTGTACAGTAA