pACYC184 Vector

Type	Cloning Vectors

GenBank	X06403

Size	4245 bp

Antibiotic selection	Chloramphenicol

Map

 

NCBI DATABASE

LOCUS       pACYC184 4245 bp    DNA SYN

DEFINITION  pACYC184

ACCESSION  

KEYWORDS   

SOURCE     

  ORGANISM  other sequences; artificial sequences; vectors.

FEATURES             Location/Qualifiers

     source           1..4245

                     /organism="pACYC184"

                     /mol_type="other DNA"

     gene            complement(4..219)

                     /label="CAT/CamR (659 - 444)"

                     /gene="CAT/CamR (659 - 444)"

     rep_origin        581..1493

                     /label="p15A_origin"

     gene            1581..2771

                     /label="tet"

                     /gene="tet"

     CDS             1581..2771

                     /label="ORF frame 3"

                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH

                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG

                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH

                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV

                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE

                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG

                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR

                     ATST*"

     misc_feature    complement(1933..1952)

                     /label="pBRrevBam_primer"

                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH

                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG

                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH

                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV

                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE

                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG

                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR

                     ATST*"

     CDS             complement(2118..2576)

                     /label="ORF frame 2"

                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ

                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER

                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"

     gene            complement(3805..219)

                     /label="CAT/CamR"

                     /gene="CAT/CamR"

                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ

                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER

                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"

     CDS             complement(3805..219)

                     /label="ORF frame 1"

                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF

                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF

                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 

ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"

                    

ORIGIN

    1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt

   61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt

  121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga

  181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga

  241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt

  301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc

  361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat

  421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt

  481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg

  541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact

  601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa

  661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc

  721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc

  781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa

  841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc

  901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc

  961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc

 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac

 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt

 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt

 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg

 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt

 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc

 1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca

 1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc

 1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct

 1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg

 1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg

 1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt

 1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc

 1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac

 1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg

 1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg

 1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt

 2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc

 2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct

 2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg

 2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg

 2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg

 2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt

 2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc

 2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg

 2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg

 2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga

 2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag

 2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca

 2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg

 2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc

 2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg

 2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg

 3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg

 3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag

 3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc

 3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta

 3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat

 3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc

 3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc

 3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac

 3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa

 3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca

 3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag

 3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg

 3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg

 3781 gcaccaataa ctgccttaa