M17 Broth

General Information

Description	For culture lactic streptococci and their bacteriophages.

Component (g/L)	Tryptone : 5 g Soy Peptone : 5 g Yeast extract : 2.5 g Meat extract : 5 g Ascorbic acid : 0.5 g Magnesium sulfate : 0.25 g Di-Sodium Glycerophosophate : 19 g Lactose : 5 g

Component (g/L)

Tryptone : 5 g

Soy Peptone : 5 g

Yeast extract : 2.5 g

Meat extract : 5 g

Ascorbic acid : 0.5 g

Magnesium sulfate : 0.25 g

Di-Sodium Glycerophosophate : 19 g

Lactose : 5 g

Protocol	Weight 42.3 g M17 Broth and mix with 1000 ml ddH₂O thoroughly. Sterilize by autoclaving at 121℃ for 15 mins. Store at 2-8℃. If there is any physical changes, discard the medium.

Storage	Room Temp and dry environment