Antibody

GAPDH pAntibody

  • Catalog Number : A1010P
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General Information

Reactivity Human, Mouse, Rabbit, Zebrafish
Application WB, IF, IHC, ELISA
Host Rabbit
Clonality Polyclonal
Conjugate Non-conjugation
Product Type Primary Antibody

Uniprot No.

P04406

Immunogen

Recombinant human Glyceraldehyde-3-phosphate dehydrogenase protein (3-105AA)
Application

WB : 1:2000-5000

IHC : 1:20-200

IF : 1:50-200

Figure

Figure 1 : Western blot analysis using GAPDH pAntibody at 2ug/ml
Lane 1: Hela whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Jurkats whole cell lysate
Lane 6: MDA-MB-231 whole cell lysate
Lane 7: LO2 whole cell lysate
Lane 8: U251 whole cell lysate
Lane 9: MCF-7 whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa

 

Figure 2 : Western Blot analysis using GAPDH pAntibody at 2.6ug/ml

Lane1 : HepG2 cell

Lane2 : Mouse brain tissue

Lane3 : Mouse skeletal muscle tissue
Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 32 kDa
Observed band size: 37 kDa

 

Figure 3 : Immunofluorescent analysis of MCF-7 cells using A1010P at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

 

Figure 4 : Immunohistochemistry of paraffin-embedded Mouse liver tissue using A1010P at dilution of 1:50

 

Figure 5 :  Immunohistochemistry of paraffin-embedded human pancreatic tissue using A1010P at dilution of 1:100

Purification Method >95%, Protein G purified
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-), GAPDH, GAPD

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