Introduction
Proliant Bovine Serum Albumin (BSA) is a high purity, lyophilized “Fraction V” powder derived from the plasma of USDA-inspected healthy animals from abattoirs located in the USA. The albumin is isolated from other plasma protein products and lipids by a unique proprietary, non-solvent procedure. The separation process includes a modified Cohn / heat-shock procedure designed to inactivate proteases and other potentially interfering enzymes. Further processing such as extensive membrane dialysis and filtration (including charcoal filtration), minimizes analytes including non-esterified fatty acids, that can cause background interference in sensitive in vitro diagnostic (IVD) assays as well as inhibition in sensitive cell and microbial culture systems. Typical purity levels are in excess of 99%. Proliant’s Reagent Grade, Fatty Acid Free albumin product is ideal for the most sensitive diagnostic and research applications.
General Information
| Storage instruction | Store sealed in a cool, dry environment for 3 years, and up to 5 years with re-qualification. |
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|
Specification |
Typical |
|
Protein (dry basis) |
≥98.0% |
> 99.0% |
|
Purity (albumin) |
≥98% |
100% |
|
Solubility (4% water) |
Clear to slightly hazy |
Clear to slightly hazy |
|
Moisture |
≤ 5.0% |
< 2.0% |
|
pH (10% water) |
6.5 to 7.5 |
6.8 to 7.2 |
|
Ash |
< 2% |
1% |
|
Protease |
≤ 0.005 Units/mg |
< 0.005 Units/mg |
|
NEFA |
< 0.01% |
< 0.005% |
|
Bovine IgG |
Not Detected |
Not Detected |
|
Endotoxin |
≤ 3 EU/mg |
≤ 0.1 EU/mg |
|
Heavy Metals (Pb) |
≤ 10 ppm |
< 1 ppm |
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