Introduction
BIS-Tris Propane is a buffer that is useful in the pH 6.3 - 9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
General Information
| Cas No. | 64431-96-5 |
|---|---|
| Purity | ≥99.0% |
| Molecular Weight | 282.33 |
| Formula | C11H26N2O6 |
| Solubility | H2O: 1 M, clear, colorless |
| Storage instruction | room temp |
| Alias | BIS-TRIS propane；1,3-Bis[tris(hydroxymethyl)methylamino]propane |
pH：10.0-12.0 (1% in H2O)
Heavy metal traces：≤0.0005%
mp ：162～165℃
Appearance: White solid
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