NFκB p65 Polyclonal Antibody
- Catalog Number : A340005
- Number : A340005-20ug
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Introduction
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, cRel,are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to
DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same
sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is
subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for
two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a
C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same
sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is
subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for
two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a
C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA, IHC-p |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugated |
| Uniprot | Q04206 |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human NFκB-p65 around the non-phosphorylation site of Ser281. |
| Assay principle | WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000 |
| Purity | Affinity purification |
| Molecular Weight | 60kDa |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Alias | RELA,NFKB3,Transcription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
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