General Information
| Application | ELISA |
|---|---|
| Assay principle | This kit uses Competitive-ELISA as the method. It can detect Ractopamine (RAC) in samples, such as muscle, feed, Liver, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been
pre-coated with coupled antigen. During the reaction, RAC in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-RAC antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of RAC. The concentration of RAC in the samples can be calculated by comparing the OD of the samples to the standard curve. |
| Sensitivity | 0.1 ppb (ng/mL) |
| Detection range | Muscle---0.4 ppb; Muscle, Liver (method 2) ---0.1 ppb; Liver---1 ppb; Feed---1 ppb; Urine ---0.1 ppb. |
| Storage instruction | Store at 2-8˚C |
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