Penicillin-Streptomycin-Amphotericin B (P/S/A) Solution (100×)
- Catalog Number : CC1055
- Number : CC1055
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Introduction
Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. Amphotericin B can bind with ergosterol on the membrane of fungi, leading to damage of the membrane, improvement of permeability, leakage of substances inside the cell, disruption of normal metabolism and bacteriostatic effect, but no effect on bacteria. The combined use of penicillin, streptomycin & amphotericin B can prevent most bacterial and fungus contamination. But penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0 - 6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable. Amphotericin B solution is unstable at room temperature, easily destroyed by light, heat and acid, and has the strongest antibacterial effect at pH 6.0 - 7.5.
General Information
| Application | Cell |
|---|---|
| Solubility | 10mM PBS (PH 6.2) |
| Storage instruction | Store at -20˚C |
INTRODUCTION:
Antibiotic-Antimycotic is a solution that is commonly added to cell culture media to prevent
contamination by various bacteria and fungi. This solution contains 10,000 units/mL of penicillin,
10,000 μg/mL of streptomycin, and 25 μg/mL of Amphotericin B. Penicillin is a Beta-lactam antibiotic
that is effective in inhibiting Gram-positive bacteria. Streptomycin is an aminoglycoside antibiotic
which is effective against most Gram-negative bacteria. Amphotericin B is effective against multicellular
fungi and yeasts.
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SPECIFICATION:
- Product Form: Liquid
- Sterility: Sterile-filtered
- Concentration: 100X
- Working concentration: 1X
- Penicillin : 10,000 units/mL
- Streptomycin: 10,000 μg/mL
- Amphotericin B: 25 μg/mL
- Antimicrobial spectrum: Gram-positive bacteria, Gram-negative bacteria & fungus
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