Introduction
This kit allows for the determination of IFN-α concentrations in Cat serum, plasma, tissue homogenates and other biological fluids.
General Information
| Reactivity | Cat |
|---|---|
| Application | ELISA |
| Assay principle | The kit assay Cat IFN-α level in the sample, use Purified Cat IFN-α antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-αto the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-αin the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Sensitivity | 1.0 pg/mL. |
| Detection range | 25 - 800 pg/mL |
| Storage instruction | Store at 2-8˚C for 6 month |
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