Nanobacteria Removal Agent (100X)
- Catalog Number : CC1053
- Number : CC1053
-
Size:
Qty : - Price : 3600 Inquiry
-
Introduction
Nanobacteria contamination is a prevalent and challenging issue in the field of cell culture. Nanobacteria can rapidly deplete arginine from the culture medium, competing with cells for essential nutrients, thereby slowing or halting cell growth. Additionally, nanobacteria can alter the enzyme profile and membrane components of cells, causing chromosomal abnormalities and cytopathological changes, which can significantly compromise the reliability of experimental results.
To address this issue, we have developed the Nanobacteria Removal Agent. This is a composite formulation specifically designed to eradicate nanobacteria contamination in cell cultures. The product contains two active components that act on nanobacteria ribosomes and DNA helicase, respectively:
1. Inhibition of Protein Synthesis: Acts on nanobacteria ribosomes, blocking protein synthesis and disrupting cell membrane integrity.
2. Inhibition of DNA Replication: Acts on nanobacteria DNA helicase, inhibiting DNA synthesis and replication.
The Nanobacteria Removal Agent. has the ability to penetrate mammalian cell membranes, allowing it to eliminate both extracellular and intracellular nanobacteria. This ensures that cells treated with the product will not be re-infected by nanobacteria released from within the cells. Within 1-2 weeks of use, the product can eradicate over 90% of nanobacteria, with minimal cytotoxic effects.
To address this issue, we have developed the Nanobacteria Removal Agent. This is a composite formulation specifically designed to eradicate nanobacteria contamination in cell cultures. The product contains two active components that act on nanobacteria ribosomes and DNA helicase, respectively:
1. Inhibition of Protein Synthesis: Acts on nanobacteria ribosomes, blocking protein synthesis and disrupting cell membrane integrity.
2. Inhibition of DNA Replication: Acts on nanobacteria DNA helicase, inhibiting DNA synthesis and replication.
The Nanobacteria Removal Agent. has the ability to penetrate mammalian cell membranes, allowing it to eliminate both extracellular and intracellular nanobacteria. This ensures that cells treated with the product will not be re-infected by nanobacteria released from within the cells. Within 1-2 weeks of use, the product can eradicate over 90% of nanobacteria, with minimal cytotoxic effects.
General Information
| Assay principle | 1. According to the characteristics of the cultured cells, the anti-nanobacteria treatment reagent is added to the corresponding complete culture medium. Prepare the fresh medium before use.
2. Recommended dilution ratio is 1:200. For example, add 50 μL of anti-nanobacteria treatment reagent to 10 mL of complete culture medium. 3. Discard the medium of cultured cells, wash the cells with sterile PBS solution, the add prepared fresh complete culture medium with anti-nanobacteria treatment reagent. The obvious effect can be seen after 3 days of continuous use. Nanobacteria can be clean up after 12-14 days of treatment. If the cell contamination is very serious, the treatment time can be extended 3-5 days appropriately. 4. Since nanobacteria may be present in the environment, in order to prevent the cells from being contaminated by the nanobacteria again, it is recommended that you continue to use antinanobacteria treatment reagent to achieve the effect of prevention. |
|---|---|
| Storage instruction | Store at -20°C. |
| Research topic | Avoid freeze-thaw. |
Usage Instructions
- Thawing Instructions: After thawing, if any crystallization occurs, place the vial in a 37°C water bath for 10-15 minutes until completely dissolved. This will not affect the product's efficacy.
- Preparation of Working Medium: Add the product to the complete culture medium at a 1:100 ratio to prepare a 1X working medium.
- Cell Passage Instructions: During cell passaging, centrifuge to remove the culture supernatant, then resuspend the cells in the 1X working medium for normal culture and passaging for 1-2 weeks. Washing cells with PBS 1-2 times during passaging can enhance the efficiency of nanobacteria elimination.
Example of Elimination Effect
.png)
Note: M represents Marker ; 1 is the positive control; 2 is the untreated nanobacteria-infected cells; 3 is the cells after one week of treatment.
.png)