ACE Script II One Step qRT-PCR Kit (SYBR)

Catalog Number : EP2028
Number : EP2028
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Introduction

The ACE Script II One Step qRT-PCR Kit (SYBR) is designed for SYBR Green I-based qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The ACE Script II Reverse Trancriptase and Champagne Taq DNA Polymerase contained in this kit and an optimized buffer system enables high-sensitive total RNA detection (as little as 1 pg). The ACE Script II One Step qRT-PCR Kit (SYBR) is a master mix system. The 2× One Step SYBR Green Mix contains an optimized buffer, dNTPs, specificityenhancer factors, and SYBR Green I. The One Step SYBR Green Enzyme Mix contains HiScript II Reverse Transcriptase, RNase inhibitors, and Champagne Taq DNA polymerase .

General Information

Application	qPCR, RT
Storage instruction	All components should be stored at -20℃

CONTENTS

Component

EP2028 250 rxn (20 μl/rxn)

RNase free ddH2O

2× One Step SYBR Green Mix ^a

One Step SYBR Green Enzyme Mix ^b

50× ROX Reference Dye 1 ^c

50× ROX Reference Dye 2 ^c

1.25 ml × 2

250 µl

100 µl

a. contains dNTP Mix, Specificity-Enhancer Factors, and SYBR Green I.

b. contains ACE Script II Reverse Transcriptase, RNase inhibitor, and Champagne Taq DNA Polymerase.

c. Used to rectify the error of fluorescence signals between different wells. Use 50× ROX Reference Dye 1 for ABI 7900HT/ 7300 Real-Time PCR and System and StepOne PlusTM；Use 50× ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System and Stratagene Mx3000P. Don't use ROX for neither Roche nor Bio-Rad Real-Time PCR instruments.

ACE Script II One Step qRT-PCR Kit (SYBR)

Introduction

General Information

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ACE Script II One Step qRT-PCR Kit (SYBR)

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